Epstein-Barr Virus

From Anthony Nolan Medical Guidelines
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Herpes virus causing glandular fever (infectious mononucleosis).

Individual at risk

Recipient (and ?donor)



Guidance at CT


Guidance at Work-up

The following serological tests should be obtained



• (EBNA-IgG)

Outcomes and recommended actions based on EBV serology and PCR:

Potential serology/PCR combinations:

1) VCA-IgM neg AND VCA-IgG pos or neg.

Donor can be cleared - transplant centre should be informed for serological/PCR status

2) VCA-IgM-pos AND VCA-IgG and/or EBNA weak or negative

a) Donor has no symptoms of viral infection* --> Inform the TC and proceed donation without EBV-DNA PCR if TC agrees. (Request EBV PCR if TC asks for it. Inform the TC with the result and act according to TC's preference)

b) Donor has symptoms of viral infection --> Request EBV DNA PCR.

- If EBV DNA PCR is positive --> inform TC and repeat PCR in 4-6 weeks 
- If EBV DNA PCR is negative --> proceed with donation when donor's symptoms get better 

  • The clinical picture (e.g. sore throat, swollen lymph glands) as well as laboratory parameters (lymphocytosis, atypical lymphocytes on blood smear) should be correlated with serology, and taken into account for reasons of donor safety. Consider more specific immunoblots to validate the serology results. Results should be communicated with transplant centre.

Justification for guidance

Primary EBV infection or reactivation in a transplant recipient is associated with post-transplant lymphoproliferative disease, amongst other morbidity.


Buisson, M., Fleurent, B., Mak, M., Morand, P., Chan, L., Ng, A., … Seigneurin, J. M. (1999). Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein-Barr Virus Primary Infection and Reactivation. Journal of Clinical Microbiology, 37(8), 2709–2714. [1]

APA Recommendations of the Center for International Blood and Marrow Transplant Research (CIBMTR®), the National Marrow Donor Program (NMDP), the European Blood and Marrow Transplant Group (EBMT), the American Society of Blood and Marrow Transplantation (ASBMT), the Canadian Blood and Marrow Transplant Group (CBMTG), the Infectious Disease Society of America (IDSA), the Society for Healthcare Epidemiology of America (SHEA), the Association of Medical Microbiology and Infectious Diseases Canada (AMMI), and the Centers for Disease Control and Prevention (CDC), Tomblyn, M., Chiller, T., Einsele, H., Gress, R., Sepkowitz, K., … Boeckh, M. A. (2009). Guidelines for Preventing Infectious Complications among Hematopoietic Cell Transplant Recipients: A Global Perspective. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation, 15(10), 1143–1238. doi:10.1016/j.bbmt.2009.06.019 [2]


Version 2, Edition 1

Date of Last Update

3 March 2016