Epstein-Barr Virus - Revision history

Maram.Ghiti@anthonynolan.org: /* Guidance at Work-up */

2025-07-31T13:48:14Z

Guidance at Work-up

← Older revision		Revision as of 13:48, 31 July 2025
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	The following serological tests should be obtained		The following serological tests should be obtained

	• VCA-IgM		• VCA-IgM - if this is positive or equivocal, an EBV PCR should be obtained.

	• VCA-IgG		• VCA-IgG
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	'''		'''

	~~Ideally, an~~ EBV PCR ~~should have been performed at medical.~~ If ~~not, TC discretion is used to guide whether an~~ EBV PCR ~~is undertaken.~~		Request EBV-DNA PCR
	If an EBV PCR ~~is not required~~, ~~a concessionary release form should~~ be ~~completed~~.
			a) If EBV-DNA PCR negative--> inform the TC and proceed donation

			b) If EBV-DNA PCR positive --> inform the TC, donation will have to be postponed and EBV serology repeated after 1 month.

Tania.Dexter@anthonynolan.org: /* Outcomes and recommended actions based on EBV serology and PCR: */

2025-06-12T09:05:18Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 09:05, 12 June 2025
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	'''		'''

	''''Donor can be cleared - transplant centre ~~should~~ be informed ~~for~~ serological~~/PCR~~ status''		''''Donor can be cleared - transplant centre will be informed of serological status on clearance document''

	''		''

Maram.Ghiti@anthonynolan.org: /* Outcomes and recommended actions based on EBV serology and PCR: */

2024-05-16T11:03:37Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 11:03, 16 May 2024
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	''		''


	2) '''VCA-IgM-pos OR equivocal AND VCA-IgG and/or EBNA positive		2) '''VCA-IgM-pos OR equivocal AND VCA-IgG and/or EBNA positive

Maram.Ghiti@anthonynolan.org: /* Guidance at Work-up */

2024-05-16T09:15:46Z

Guidance at Work-up

← Older revision		Revision as of 09:15, 16 May 2024
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	''		''

	2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive		2) '''VCA-IgM-pos OR equivocal AND VCA-IgG and/or EBNA positive
	'''		'''

	~~Inform the~~ TC ~~and proceed donation without~~ EBV~~-DNA~~ PCR		Ideally, an EBV PCR should have been performed at medical. If not, TC discretion is used to guide whether an EBV PCR is undertaken.
			If an EBV PCR is not required, a concessionary release form should be completed.


	3)'''VCA-IgM-pos AND VCA-IgG and/or EBNA weak or negative		3)'''VCA-IgM-pos OR equivocal AND VCA-IgG and/or EBNA weak or negative

	Request EBV-DNA PCR		Request EBV-DNA PCR

MariaCuadrado: /* Outcomes and recommended actions based on EBV serology and PCR: */

2020-01-10T14:22:18Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 14:22, 10 January 2020
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	'''		'''

	''Donor can be cleared - transplant centre should be informed for serological/PCR status''		''''Donor can be cleared - transplant centre should be informed for serological/PCR status''


			''

	2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive		2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive

MariaCuadrado: /* Outcomes and recommended actions based on EBV serology and PCR: */

2020-01-10T14:21:53Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 14:21, 10 January 2020
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	2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive		2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive
	'''		'''

	Inform the TC and proceed donation without EBV-DNA PCR		Inform the TC and proceed donation without EBV-DNA PCR

MariaCuadrado: /* Outcomes and recommended actions based on EBV serology and PCR: */

2020-01-10T14:21:15Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 14:21, 10 January 2020
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	2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA ~~weak or negative~~		2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA positive
	'''		'''
			Inform the TC and proceed donation without EBV-DNA PCR

	a) Donor has no symptoms of viral infection* --> Inform the TC and proceed donation without EBV-DNA PCR if TC agrees. (Request EBV PCR if TC asks for it. Inform the TC with the result and act according to TC's preference)

	b) ~~Donor has symptoms of viral infection --> Request EBV DNA PCR.~~		3)'''VCA-IgM-pos AND VCA-IgG and/or EBNA weak or negative
	- ~~If EBV DNA PCR is positive~~ --~~> inform TC~~ and ~~repeat PCR in 4-6 weeks~~
	~~- If EBV DNA PCR is~~ negative ~~--> proceed with donation when donor's symptoms get better~~

			Request EBV-DNA PCR

			a) If EBV-DNA PCR negative--> inform the TC and proceed donation

	*The clinical picture (e.g. sore throat, swollen lymph glands) ~~as well as laboratory parameters (lymphocytosis~~, ~~atypical lymphocytes on blood smear) should~~ be ~~correlated with serology,~~ and ~~taken into account for reasons of donor safety. Consider more specific immunoblots to validate the~~ serology ~~results. Results should be communicated with transplant centre~~.''		b) If EBV-DNA PCR positive --> inform the TC, donation will have to be postponed and EBV serology repeated after 1 month.

	==Justification for guidance==		==Justification for guidance==

MariaCuadrado: /* Guidance at Work-up */

2018-11-26T17:39:39Z

Guidance at Work-up

← Older revision		Revision as of 17:39, 26 November 2018
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	• VCA-IgG		• VCA-IgG

	• (EBNA-IgG)		• (EBNA-IgG)


	~~If VCA-IgM is positive, EBV-PCR should be undertaken~~

	===Outcomes and recommended actions based on EBV serology and PCR:===		===Outcomes and recommended actions based on EBV serology and PCR:===
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	1) VCA-IgM neg AND VCA-IgG pos or neg.		1) '''VCA-IgM neg AND VCA-IgG pos or neg.

	OR

	~~VCA-IgM-pos AND VCA-IgG pos AND EBV-PCR = negative~~

			'''

	''Donor can be cleared - transplant centre should be informed for serological/PCR status''		''Donor can be cleared - transplant centre should be informed for serological/PCR status''
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	2) VCA-IgM-pos AND VCA-IgG and/or EBNA weak or negative ~~AND EBV-PCR=negative~~		2) '''VCA-IgM-pos AND VCA-IgG and/or EBNA weak or negative
			'''
	'~~'IgM could be non-specific or donor has acute (inapperent) infection~~

	~~Donor can be cleared.~~

	The clinical picture (e.g. sore throat, swollen lymph glands) as well as laboratory parameters (lymphocytosis, atypical lymphocytes on blood smear) should be correlated with serology, and taken into account for reasons of donor safety. Consider more specific immunoblots to validate the serology results. Results should be communicated with transplant centre.''

			a) Donor has no symptoms of viral infection* --> Inform the TC and proceed donation without EBV-DNA PCR if TC agrees. (Request EBV PCR if TC asks for it. Inform the TC with the result and act according to TC's preference)

			b) Donor has symptoms of viral infection --> Request EBV DNA PCR.
			- If EBV DNA PCR is positive --> inform TC and repeat PCR in 4-6 weeks
			- If EBV DNA PCR is negative --> proceed with donation when donor's symptoms get better

	~~3) EBV-PCR = positive~~


	~~''Donor cannot~~ be ~~cleared~~. ~~Inform~~ transplant centre ~~and repeat EBV-PCR in 4-6 weeks~~.		*The clinical picture (e.g. sore throat, swollen lymph glands) as well as laboratory parameters (lymphocytosis, atypical lymphocytes on blood smear) should be correlated with serology, and taken into account for reasons of donor safety. Consider more specific immunoblots to validate the serology results. Results should be communicated with transplant centre.''

	==Justification for guidance==		==Justification for guidance==

PaulM at 08:49, 16 April 2018

2018-04-16T08:49:19Z

← Older revision		Revision as of 08:49, 16 April 2018
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	''IgM could be non-specific or donor has acute (inapperent) infection		''IgM could be non-specific or donor has acute (inapperent) infection

			Donor can be cleared.

	The clinical picture (e.g. sore throat, swollen lymph glands) as well as laboratory parameters (lymphocytosis, atypical lymphocytes on blood smear) should be correlated with serology, and taken into account for reasons of donor safety. Consider more specific immunoblots to validate the serology results. Results should be communicated with transplant centre.''		The clinical picture (e.g. sore throat, swollen lymph glands) as well as laboratory parameters (lymphocytosis, atypical lymphocytes on blood smear) should be correlated with serology, and taken into account for reasons of donor safety. Consider more specific immunoblots to validate the serology results. Results should be communicated with transplant centre.''
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	''Donor cannot be cleared ~~at the current timepoint~~. Inform transplant centre and repeat EBV-PCR in ~~one month~~		''Donor cannot be cleared. Inform transplant centre and repeat EBV-PCR in 4-6 weeks.

	==Justification for guidance==		==Justification for guidance==

PaulM: /* Outcomes and recommended actions based on EBV serology and PCR: */

2017-11-01T09:45:19Z

Outcomes and recommended actions based on EBV serology and PCR:

← Older revision		Revision as of 09:45, 1 November 2017
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	''Donor cannot be cleared at the current timepoint. Inform transplant centre and repeat EBV-PCR in one month"		''Donor cannot be cleared at the current timepoint. Inform transplant centre and repeat EBV-PCR in one month

	==Justification for guidance==		==Justification for guidance==